International Journal of Clinical Research

Objective To evaluate the quantitative differential diagnostic value of Syncytin-1 RNA expression in bile exosomes for patients with cholangiocarcinoma and cholelithiasis. Methods The bile samples of 30 patients with newly diagnosed cholangiocarcinoma (CCA) in the body fluid specimen bank of our hospital from March 2021 to November 2021 were retrospectively selected to form the CCA group, and the bile samples of 30 patients with confirmed cholelithiasis were selected to form the cholelithiasis group.Bile exosomes were extracted by exosome peecipitation solution ; RNA extraction, reverse transcription and real-time fluorescent PCR ( RT-PCR ) were used to detect the expression level of Syncytin-1 RNA in bile exosomes. The expression levels of Syncytin-1 RNA in bile sample exosomes between groups were compared to evaluate the quantitative differential diagnosis efficacy of the above indicator for cholangiocarcinoma and cholelithiasis. The linear correlation between the aboveindicator and the levels of tumor-associated glycoprotein (carcinoembryonic antigen and serum carbohydrate antigen 724) in bile was evaluated. Results The differences in the average CT values of Syncytin-1 RNA between the two groups were statistically significant (P< 0.05). The above indicator had high quantitative differential diagnosis efficacy for cholangiocarcinoma and cholelithiasis, with AUCs of 0.919, respectively. Diagnostic thresholds were26.20, respectively.The above indexes were moderately positively correlated with bile carcinoembryonic antigen and serum carbohydrate antigen 724 levels. Conclusion The expression of Syncytin-1 RNA in bile exosomes has certain quantitative diagnostic value for cholangiocarcinoma and cholelithiasis, and has certain correlation with some tumor-associated glycoproteins in bile, which provides more evidence for the diagnosis and differential diagnosis of cholangiocarcinoma.

Bile; Exosomes; Syncytin-1; Cholangiocarcinoma; Cholelithiasis; Differential Diagnosis