Understanding the Physiological and Transcriptional Mechanism of Reproductive Stage Soybean in Response to Heat Stress-Crop Breeding, Genetics and Genomics-CSCIED科技核心评价数据库-手机版

Understanding the Physiological and Transcriptional Mechanism of Reproductive Stage Soybean in Response to Heat Stress

Understanding the Physiological and Transcriptional Mechanism of Reproductive Stage Soybean in Response to Heat Stress

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DOI	10.20900/cbgg20200004
刊名	Crop Breeding, Genetics and Genomics CBGG
年，卷(期)	2020, 2(1)
作者	Congshan Xu,Zhiqiang Xia,Zhiqiang Huang,Chao Xia,Jing Huang,Manrong Zha,Shujuan Wang,Muhammad Imran,Shaun Casteel,Yiwei Jiang,Cankui Zhang*,
作者单位
Abstract	Understanding the physiological and molecular processes in response to heat in soybean is important to implement strategies to breed more heat tolerant soybean. In this study, physiological and RNA-Seq analysis were used to dissect the physiological alterations and molecular responses in the leaves of reproductive stage soybean grown at heat condition. Stomata conductance and transpiration rate were increased but photosynthesis and relative water content were reduced in response to heat. Among the three measured antioxidant enzymes, the activity of peroxidase was increased but no changes were detected for catalase and superoxide dismutase. A total of 633 annotated genes were found to be differentially expressed in heat stressed plants in which the expression levels of 417 genes were up-regulated and 216 were down-regulated. Most dramatic responses are related to flowering, oxidative stress, protein and mRNA folding and degradation, protective compounds synthesis, and ethylene biosynthesis and signaling. The diverse pathways identified in this study indicated that soybean employs complicated mechanisms to cope with heat. A few of the identified genes and pathways may be manipulated to improve heat resistance ability in soybean via either genetic engineering or molecular breeding method.
KeyWord	soybean; heat; transcriptome; reproductive stage
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相关文献

1.Hall AE. Breeding cowpea for future climates. In: Yadav SS, Redden RJ, Hatfield JL, Lotze-Campen H, editors. Crop adaptation to climate change. Hoboken (US): John Wiley & Sons; 2011.

2.Rahmstorf S, Coumou D. Increase of extreme events in a warming world. Proc Natl Acad Sci U S A. 2011;108:17905-9. doi: 10.1073/pnas.1101766108

3.Wahid A, Shabbir A. Induction of heat stress tolerance in barley seedlings by pre-sowing seed treatment with glycinebetaine. Plant Growth Regul. 2005;46(2):133-41. doi: 10.1007/s10725-005-8379-5

4.Hatfield JL, Prueger JH. Temperature extremes: Effect on plant growth and development. Weather Clim Extremes. 2015;10:4-10. doi: 10.1016/j.wace.2015.08.001

5.Lafta AM, Lorenzen JH. Effect of high temperature on plant growth and carbohydrate metabolism in potato. Plant Physiol. 1995;109(2):637-43. doi: 10.1104/pp.109.2.637

6.Omae H, Kumar A, Shono M. Adaptation to high temperature and water deficit in the common bean (Phaseolus vulgaris L.) during the reproductive period. J Bot. 2012;2012:803413. doi: 10.1155/2012/803413

7.Bañon S, Fernandez JA, Franco JA, Torrecillas A, Alarcón JJ, Sánchez-Blanco MJ. Effects of water stress and night temperature preconditioning on water relations and morphological and anatomical changes of Lotus creticus plants. Sci Hortic. 2004;101(3):333-42. doi: 10.1016/j.scienta.2003.11.007

8.Tan W, Meng QW, Brestic M, Olsovska K, Yang X. Photosynthesis is improved by exogenous calcium in heat-stressed tobacco plant. J Plant Physiol. 2011;168(17):2063-71. doi: 10.1016/j.jplph.2011.06.009

9.Bohnert HJ, Gong Q, Li P, Ma S. Unraveling abiotic stress tolerance mechanisms—getting genomics going. Curr Opin Plant Biol. 2006;9(2):180-8. doi: 10.1016/j.pbi.2006.01.003

10.Vinocur B, Altman A. Recent advances in engineering plant tolerance to abiotic stress: achievements and limitations. Curr Opin Biotechnol. 2005;16(2):123-32. doi: 10.1016/j.copbio.2005.02.001

11.Bonos SA, Murphy JA. Growth responses and performance of Kentucky bluegrass under summer stress. Crop Sci. 1999;39(3):770-4. doi: 10.2135/cropsci1999.0011183X003900030026x

12.Adams SR, Cockshull KE, Cave CRJ. Effect of temperature on the growth and development of tomato fruits. Ann Bot. 2001;88(5):869-77. doi: 10.1006/anbo.2001.1524

13.Valdés-López O, Batek J, Gomez-Hernandez N, Nguyen CT, Isidra-Arellano MC, Zhang N, et al. Soybean roots grown under heat stress show global changes in their transcriptional and proteomic profiles. Front Plant Sci. 2016;7:517.doi: 10.3389/fpls.2016.00517

14.Das A, Eldakak M, Paudel B, Kim DW, Hemmati H, Basu C, et al. Leaf proteome analysis reveals prospective drought and heat stress response mechanisms in soybean. BioMed Res Int. 2016;2016:6021047. doi: 10.1155/2016/6021047

15.Wang L, Liu L, Ma Y, Li S, Dong S, Zu W. Transcriptome profilling analysis characterized the gene expression patterns responded to combined drought and heat stresses in soybean. Comput Biol Chem. 2018;77:413-29. doi: 10.1016/j.compbiolchem.2018.09.012

16.Mittler R, Zilinskas BA. Molecular cloning and characterization of a gene encoding pea cytosolic ascorbate peroxidase. J Biol Chem. 1992;267(30):21802-7. doi: 10.1016/0014-5793(92)81127-8

17.Xu C, Xia C, Xia Z, Zhou X, Huang J, Huang Z, et al. Physiological and transcriptomic responses of reproductive stage soybean to drought stress. Plant Cell Rep. 2018;37(12):1611-24. doi: 10.1007/s00299-018-2332-3

18.Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72(1-2):248-54.

19.Kim D, Pertea G, Trapnell C, Pimentel H, Kelley R, Salzberg SL. TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions. Genome Biol. 2013;14(4):R36. doi: 10.1186/gb-2013-14-4-r36

20.Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B. Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods. 2008;5(7):621. doi: 10.1038/nmeth.1226

21.Dutta S, Mohanty S, Tripathy BC. Role of temperature stress on chloroplast biogenesis and protein import in pea. Plant Physiol. 2009;150(2):1050-61.doi: 10.1104/pp.109.137265

22.Li D, Ruan X, Xu Q, Wang K, Gong Y, Kuang T, et al. Heat denaturation of protein structures and chlorophyll states in PSII membranes. Tsinghua Sci Technol. 2002;7(4):407-10. doi: 10.1007/BF03030694

23.Tripathy BC, Oelmüller R. Reactive oxygen species generation and signaling in plants. Plant Signal Behav. 2012;7(12):1621-33. doi: 10.4161/psb.22455

24.You J, Chan Z. ROS regulation during abiotic stress responses in crop plants. Front Plant Sci. 2015;6:1092. doi: 10.3389/fpls.2015.01092

25.Zhao X, Huang L, Zhang X, Li Z, Peng Y. Effects of heat acclimation on photosynthesis, antioxidant enzyme activities, and gene expression in orchard grass under heat stress. Molecules. 2014;19(9):13564-76. doi: 10.3390/molecules190913564

26.Ara N, Nakkanong K, Lv W, Yang J, Hu Z, Zhang M. Antioxidant enzymatic activities and gene expression associated with heat tolerance in the stems and roots of two cucurbit species (“Cucurbita maxima” and “Cucurbita moschata”) and their interspecific inbred line “Maxchata”. Int J Mol Sci. 2013;14(12):24008-28. doi: 10.1016/j.envexpbot.2012.09.009

27.Lee JH, Ryu HS, Chung KS, Posé D, Kim S, Schmid M, et al. Regulation of temperature-responsive flowering by MADS-box transcription factor repressors. Science. 2013;342(6158):628-32. doi: 10.1126/science.1241097

28.Nakano Y, Higuchi Y, Sumitomo K, Hisamatsu T. Flowering retardation by high temperature in chrysanthemums: involvement of FLOWERING LOCUS T-like 3 gene repression. J Exp Bot. 2013;64(4):909-20. doi: 10.1093/jxb/ers370

29.Kazan K, Lyons R. The link between flowering time and stress tolerance. J Exp Bot. 2015;67(1):47-60. doi: 10.1093/jxb/erv441

30.Fujiwara S, Oda A, Yoshida R, Niinuma K, Miyata K, Tomozoe Y, et al. Circadian clock proteins LHY and CCA1 regulate SVP protein accumulation to control flowering in Arabidopsis. Plant Cell. 2008;20(11):2960-71. doi: 10.1105/tpc.108.061531

31.Riboni M, Robustelli Test A, Galbiati M, Tonelli C, Conti L. Environmental stress and flowering time: the photoperiodic connection. Plant Signal Behav. 2014;9(7):e29036. doi: 10.4161/psb.29036

32.Shim JS, Kubota A, Imaizumi T. Circadian clock and photoperiodic flowering in Arabidopsis: CONSTANS is a hub for signal integration. Plant Physiol. 2017;173(1):5-15. doi: 10.1104/pp.16.01327

33.Song YH, Estrada DA, Johnson RS, Kim SK, Lee SY, MacCoss MJ, et al. Distinct roles of FKF1, GIGANTEA, and ZEITLUPE proteins in the regulation of CONSTANS stability in Arabidopsis photoperiodic flowering. Proc Natl Acad Sci U S A. 2014;111(49):17672-7. doi: 10.1073/pnas.1415375111

34.Yang Y, Peng Q, Chen GX, Li XH, Wu CY. OsELF3 is involved in circadian clock regulation for promoting flowering under long-day conditions in rice. Mol Plant. 2013;6(1):202-15. doi: 10.1093/mp/sss062

35.Klocko AL, Ma C, Robertson S, Esfandiari E, Nilsson O, Strauss SH. FT overexpression induces precocious flowering and normal reproductive development in Eucalyptus. Plant Biotechnol J. 2016;14(2):808-19. doi: 10.1111/pbi.12431

36.Tränkner C, Lehmann S, Hoenicka H, Hanke MV, Fladung M, Lenhardt D, Flachowsky H. Over-expression of an FT-homologous gene of apple induces early flowering in annual and perennial plants. Planta. 2010;32(6):1309-24. doi: 10.1007/s00425-010-1254-2

37.Yoo SK, Chung KS, Kim J, Le JH, Hong SM, Yoo SJ, et al. Constans activates suppressor of overexpression of constans 1 through Flowering Locus T to promote flowering in Arabidopsis. Plant Physiol. 2005;139(2):770-8. doi: 10.2307/4281911

38.Chen Q, Payyavula RS, Chen L, Zhang J, Zhang C, Turgeon R. FLOWERING LOCUS T mRNA is synthesized in specialized companion cells in Arabidopsis and Maryland Mammoth tobacco leaf veins. Proc Natl Acad Sci U S A. 2018;115(11):2830-5. doi: 10.1073/pnas.1719455115

39.Park MJ, Kwon YJ, Gil KE, Park CM. LATE ELONGATED HYPOCOTYL regulates photoperiodic flowering via the circadian clock in Arabidopsis. BMC Plant Biol. 2016;16(1):114. doi: 10.1186/s12870-016-0810-8

40.Small ID, Peeters N. The PPR motif—a TPR-related motif prevalent in plant organellar proteins. Trends Biochem Sci. 2000;25(2):45-7. doi: 10.1016/S0968-0004(99)01520-0

41.Schmitz-Linneweber C, Small I. Pentatricopeptide repeat proteins: a socket set for organelle gene expression. Trends Plant Sci. 2008;13(12):663-70. doi: 10.1016/j.tplants.2008.10.001

42.Zsigmond L, Rigó G, Szarka A, Székely G, Ötvös K, Darula Z, et al. Arabidopsis PPR40 connects abiotic stress responses to mitochondrial electron transport. Plant Physiol. 2008;146(4):1721-37. doi: 10.1104/pp.107.111260

43.Harsh A, Sharma YK, Joshi U, Rampuria S, Singh G, Kumar S, et al. Effect of short-term heat stress on total sugars, proline and some antioxidant enzymes in moth bean (Vigna aconitifolia). Ann Agr Sci. 2016;61(1):57-64. doi: 10.1016/j.aoas.2016.02.001

44.Giri J. Glycinebetaine and abiotic stress tolerance in plants. Plant Signal Behav. 2011;6(11):1746-51.

45.Heuer B. Influence of exogenous application of proline and glycinebetaine on growth of salt-stressed tomato plants. Plant Sci. 2003;165(4):693-9. doi: 10.1016/s0168-9452(03)00222-x

46.Jennings DH. Compatible solutes—the mycological dimension and their role as physiological buffering agents. New Phytol. 1990;116(2):277-83. doi: 10.1111/j.1469-8137.1990.tb04715.x

47.Slama I, Abdelly C, Bouchereau A, Flowers T, Savoure A. Diversity, distribution and roles of osmoprotective compounds accumulated in halophytes under abiotic stress. Ann Bot. 2015;115(3):433-47.

48.Song J, Xing Y, Munir S, Yu C, Song L, Li H, et al. An ATL78-Like RING-H2 finger protein confers abiotic stress tolerance through interacting with RAV2 and CSN5B in tomato. Front Plant Sci. 2016;7:1305. doi: 10.3389/fpls.2016.01305

49.Wu W, Cheng Z, Liu M, Yang X, Qiu D. C3HC4-type RING finger protein NbZFP1 is involved in growth and fruit development in Nicotiana benthamiana. PLoS One. 2014;9(6):e99352. doi: 10.1371/journal.pone.0099352

50.Yu Y, Xu W, Wang S, Xu Y, Li HE, Wang Y, et al. VpRFP1, a novel C4C4-type RING finger protein gene from Chinese wild Vitis pseudoreticulata, functions as a transcriptional activator in defence response of grapevine. J Exp Bot. 2011;62(15):5671-82. doi: 10.1093/jxb/err253

51.Zang D, Li H, Xu H, Zhang W, Zhang Y, Shi X, et al. An Arabidopsis zinc finger protein increases abiotic stress tolerance by regulating sodium and potassium homeostasis, reactive oxygen species scavenging and osmotic potential. Front Plant Sci. 2016;7:1272.

52.Verslues PE, Sharma S. Proline metabolism and its implications for plant-environment interaction. Arabidopsis Book. 2010;8:e0140. doi: 10.1199/tab.0140

53.Kalamaki MS, Alexandrou D, Lazari D, Merkouropoulos G, Fotopoulos V, Pateraki I, et al. Over-expression of a tomato N-acetyl-l-glutamate synthase gene (SlNAGS1) in Arabidopsis thaliana results in high ornithine levels and increased tolerance in salt and drought stresses. J Exp Bot. 2009;60(6):1859-71. doi: 10.1093/jxb/erp072

54.Whitley D, Goldberg SP, Jordan WD. Heat shock proteins: a review of the molecular chaperones. J Vasc Surg. 1999;29(4):748-51. doi: 10.5423/ppj.rw.08.2015.0150

55.Yan J, Wang J, Li Q, Hwang JR, Patterson C, Zhang H. AtCHIP, a U-box-containing E3 ubiquitin ligase, plays a critical role in temperature stress tolerance in Arabidopsis. Plant Physiol. 2003;132(2):861-9. doi: 10.2307/4281161

56.Tanner NK, Linder P. DExD/H box RNA helicases: from generic motors to specific dissociation functions. Mol Cell. 2001;8(2):251-62. doi: 10.1016/S1097-2765(01)00329-X

57.Lorenzo O, Piqueras R, Sánchez-Serrano JJ, Solano R. ETHYLENE RESPONSE FACTOR1 integrates signals from ethylene and jasmonate pathways in plant defense. Plant Cell. 2003;15(1):165-78. doi: 10.1105/tpc.007468.signaling

58.Pré M, Atallah M, Champion A, De Vos M, Pieterse CM, Memelink J. The AP2/ERF domain transcription factor ORA59 integrates jasmonic acid and ethylene signals in plant defense. Plant Physiol. 2008;147(3):1347-57. doi: 10.1104/pp.108.117523

59.Raghavan C, Ong EK, Dalling MJ, Stevenson TW. Regulation of genes associated with auxin, ethylene and ABA pathways by 2,4-dichlorophenoxyacetic acid in Arabidopsis. Funct Integr Genom. 2006;6(1):60-70. doi: 10.1007/s10142-005-0012-1

60.Shinshi H. Ethylene-regulated transcription and crosstalk with jasmonic acid. Plant Sci. 2008;175(1-2):18-23. doi: 10.1016/j.plantsci.2008.03.017

61.Lu X, Jiang W, Zhang L, Zhang F, Shen Q, Wang T, et al. Characterization of a novel ERF transcription factor in Artemisia annua and its induction kinetics after hormones and stress treatments. Mol Biol Rep. 2012;39(10):9521-7. doi: 10.1007/s11033-012-1816-4

62.Pan Y, Seymour GB, Lu C, Hu Z, Chen X, Chen G. An ethylene response factor (ERF5) promoting adaptation to drought and salt tolerance in tomato. Plant Cell Rep. 2012;31(2):349-60. doi: 10.1007/s00299-011-1170-3

63.Shi Q, Dong Y, Zhou Q, Qiao D, Ma Z, Zhang L, et al. Characterization of a maize ERF gene, ZmERF1, in hormone and stress responses. Acta Physiol Plant. 2016;38(5):126. doi: 10.1007/s11738-016-2146-2

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Congshan Xu,Zhiqiang Xia,Zhiqiang Huang,Chao Xia,Jing Huang,Manrong Zha,Shujuan Wang,Muhammad Imran,Shaun Casteel,Yiwei Jiang,Cankui Zhang*. Understanding the Physiological and Transcriptional Mechanism of Reproductive Stage Soybean in Response to Heat Stress, Crop Breeding, Genetics and Genomics. 2020; 2; (1). https://doi.org/10.20900/cbgg20200004.

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