染料木素抗PC12神经细胞炎症作用实验研究-国际中医药研究-CSCIED科技核心评价数据库-手机版

染料木素抗PC12神经细胞炎症作用实验研究

Experimental Study on the Anti inflammatory Effect of Genistein on PC12 Nerve Cells

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DOI	10.12208/j.ircm.20220080
刊名	国际中医药研究 International Research in Chinese Medicine
年，卷(期)	2022, 2(4)
作者	周迎*,熊婷,陈莹,李海刚,
作者单位	长沙医学院药学院湖南长沙 ;
摘要	探讨染料木素对双氧水诱导的PC12细胞炎症模型的影响及其抗炎作用机制。方法 1、筛选适合造模的双氧水浓度构建PC12细胞炎症模型。2、测定不同浓度染料木素对细胞存活率的影响。3、MTT法观测染料木素对PC12细胞双氧水炎症模型的预保护作用。4、实时PCR检测各组PC12细胞中NgR mRNA的表达。结果 1、染料木素浓度100 μM以下基本不具细胞毒性，染料木素预处理具有保护作用，与模型组相比显著提高PC12炎症模型细胞存活率。2、染料木素显著下调H2O2损伤引起NgR基因的表达。结论染料木素可通过Nogo/NgR信号通路，下调NgR水平发挥抗PC12神经细胞炎症的作用。
Abstract	Objective To To investigate the effect of genistein on PC12 cell inflammatory model induced by hydrogen peroxide and its anti-inflammatory mechanism. Methods 1. Select the suitable concentration of hydrogen peroxide for modeling to construct PC12 cell inflammation model. 2. The effects of different concentrations of genistein on cell survival were determined. 3. MTT assay was used to observe the protective effect of genistein on PC12 cells with H2O2 induced inflammation. 4. The expression of NgR mRNA in PC12 cells of each group was detected by real-time PCR. Result 1. Genistein concentration 100 μ There is basically no cytotoxicity below M, and genistein pretreatment has a protective effect. Compared with the model group, it significantly improves the cell survival rate of PC12 inflammatory model. 2. Genistein significantly decreased the expression of NgR gene induced by H2O2 damage. Conclusion Genistein can inhibit the inflammation of PC12 neurons by down regulating the level of NgR through Nogo/NgR signaling pathway.
关键词	染料木素；神经炎症；抗炎；NgR
KeyWord	genistein; Neuroinflammation; Anti inflammation; NgR
基金项目
页码	26-28

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引用本文

周迎*,熊婷,陈莹,李海刚. 染料木素抗PC12神经细胞炎症作用实验研究 [J]. 国际中医药研究. 2022; 2; (4). 26 - 28.

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