亲环素A在巨噬细胞荷脂过程中的影响-CSCIED科技核心评价数据库-手机版

亲环素A在巨噬细胞荷脂过程中的影响

Effect of Cyclophilin A on Cellular Cholesterol Accumulation in Lipid-Loaded Macrophages Induced by Oxidized Low Density Lipoprotein

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刊名	中国动脉硬化杂志
年，卷(期)	2005, (05)
作者	涂剑1严鹏科1王北冰2曹轩1王蓉蓉1廖端芳1,
作者单位	1.南华大学药物药理研究所2.南华大学诊断学教研室
摘要	论文观察胆固醇转运复合物中关键蛋白亲环素A在氧化型低密度脂蛋白诱导的巨噬细胞荷脂过程中的表达变化及其与细胞内胆固醇蓄积的关系,进而分析亲环素A在巨噬源性泡沫细胞形成过程中的作用。结果表明，75 mg/L氧化型低密度脂蛋白与RAW264.7细胞共同孵育48 h后,亲环素A的蛋白表达逐渐减弱,48 h、72 h分别较未处理组下降了54.5%±6.3%、59.8%±5.9%(P<0.05)。间接免疫荧光定位检测发现氧化型低密度脂蛋白显著抑制RAW264.7细胞中亲环素A在胞膜、胞浆中的表达,且随着处理时间的延长作用更明显。细胞内胆固醇酯/总胆固醇的比值由未处理组的28.3%±1.2%上升至48 h的42.3%±5.9%(P均<0.05)。
Abstract	This study investigated the expression changes of Cyclophilin A, a key protein in the cholesterol transport complex, during oxidized low-density lipoprotein (ox-LDL)-induced lipid loading in macrophages and its relationship with intracellular cholesterol accumulation. The aim was to analyze the role of Cyclophilin A in the formation of macrophage-derived foam cells. The results showed that after incubating RAW264.7 cells with 75 mg/L ox-LDL for 48 hours, the protein expression of Cyclophilin A gradually decreased. At 48 hours and 72 hours, the expression levels decreased by 54.5% ± 6.3% and 59.8% ± 5.9%, respectively, compared to the untreated group (P < 0.05). Indirect immunofluorescence localization assays revealed that ox-LDL significantly suppressed the expression of Cyclophilin A in both the cell membrane and cytoplasm of RAW264.7 cells, with the inhibitory effect becoming more pronounced over time. The ratio of intracellular cholesteryl ester to total cholesterol increased from 28.3% ± 1.2% in the untreated group to 42.3% ± 5.9% at 48 hours (P < 0.05).
关键词	病理学与病理生理学;亲环素A;巨噬细胞;荷脂化;脂蛋白,低密度,氧化;胆固醇蓄积;Westernblot法;高效液相色谱法;
KeyWord	Cyclophilin A;RAW264.7 Macrophages;Lipid-Loaded;Oxidized Low Density Lipoprotein;Cholesterol Accumulation
基金项目
页码	541-544

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引用本文

涂剑严鹏科王北冰曹轩王蓉蓉廖端芳. 亲环素A在巨噬细胞荷脂过程中的影响 [J]. 中国动脉硬化杂志. 2005; ; (05). 541 - 544.

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