过表达外源M-CSF促进MCF7细胞增殖-CSCIED科技核心评价数据库-手机版

过表达外源M-CSF促进MCF7细胞增殖

Overexpression of M-CSF Promotes Proliferation of MCF7 Cells

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DOI
刊名	中国生物化学与分子生物学报
年，卷(期)	2009, 25(02)
作者	涂剑1姚志红1龙治峰2朱炳阳1唐圣松1,
作者单位	1.南华大学药物药理研究所2.南华大学组胚学教研室
摘要	为探讨过表达外源巨噬细胞集落刺激因子(M-CSF)对细胞增殖的影响,将重组载体pCMV/cyto/myc-M-CSF转染MCF7细胞、G418筛选,RT-PCR、Western印迹和免疫荧光鉴定M-CSF的mRNA表达、蛋白表达及定位,通过计算细胞倍增时间、MTT法及反义寡核苷酸抑制实验分析M-CSF对细胞增殖的影响.结果表明,转染M-CSF的MCF7细胞过表达M-CSF mRNA及蛋白,并且定位表达于细胞质;与转染空载体及未转染M-CSF组细胞比较,转染M-CSF的MCF7细胞倍增时间明显缩短、增殖能力显著增强,M-CSF的特异性反义寡核苷酸能抑制转染M-CSF的MCF7细胞的增殖,且抑制率随着反义寡核苷酸浓度的增高而增强;以上结果提示,胞质过表达M-CSF可促进MCF7细胞的增殖.
Abstract	To explore the effect of macrophage colony stimulating factor(M-CSF) on the cell proliferation,pCMV/cyto/myc-M-CSF vectors were transfected into MCF7 cells using liposomes and screened with G418.RT-PCR,Western blot and immunofluorescence assays were performed to verify the M-CSF mRNA or protein expressions and localization.The results indicated that a high level of M-CSF expression in the cytoplasm was detected which promoted cell proliferation as shown by cell doubling time,MTT assays in M-CSF-transfected MCF7 cells.In addition,the proliferation of MCF7 cells transfected with M-CSF-specific antisense oligonucleotides was significantly inhibited than those of pCMV/cyto/myc transfected or untransfected MCF7 cells.We conclude that the overexpression of M-CSF promotes the proliferation of MCF7 cells.
关键词	巨噬细胞集落刺激因子;pCMV/cyto/myc-M-CSF载体;MCF7细胞;胞质;细胞增殖;
KeyWord	macrophage colony stimulating factor(M-CSF);pCMV/cyto/myc-M-CSF vector;MCF7 cells;cytoplasm;cell proliferation
基金项目
页码	132-136

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引用本文

涂剑姚志红龙治峰朱炳阳唐圣松. 过表达外源M-CSF促进MCF7细胞增殖 [J]. 中国生物化学与分子生物学报. 2009; 25; (02). 132 - 136.

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