Journal of Food Safety and Quality

Objective To establish a method for the determination of 8 kinds of benzodiazepines residues, including diazepam, oxazepam, midazolam, clonazepam, lorazepam, triazolam, alprazolam and nitrazepam in aquatic products by ultra performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS). Methods The sample was extracted by 80% acetonitrile aqueous solution containing 0.2% formic acid, purified by Waters PRIME HLB solid phase extraction column, concentrated by nitrogen blowing in water bath. The residue contents were separated by C18 chromatographic column, eluted by 0.1% formic acid aqueous solution-methanol mobile phase systems, and analyzed by electrospray ion source in positive ion multi-reaction monitoring mode. The quantitative information was obtained by matrix-added calibration with external standard. Results The correlation coefficient of calibration curves of 8 kinds of benzodiazepines residues were above 0.999. The limits of detection were 0.064–0.226 μg/kg, the recovery rates of the method were 70.4%–107.0%, and the relative standard deviations were 2.1%–10.2%. Conclusion The method is rapid, simple and accurate, and it is suitable for the determination of benzodiazepine sedative drug residues in aquatic products.

aquatic products; drug residues; benzodiazepines; sedativetetracyclines; ultra performance liquid chromatography-tandem mass spectrometry