基于大豆过敏蛋白的植物替代蛋白产品真实性研究-食品安全质量检测学报-CSCIED科技核心评价数据库-手机版

基于大豆过敏蛋白的植物替代蛋白产品真实性研究

Study on the authenticity determination of plant-based proteins based on soybean allergens

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DOI	10.19812/j.cnki.jfsq11-5956/ts.20241224001
刊名	食品安全质量检测学报 Journal of Food Safety and Quality
年，卷(期)	2025, 16(12)
作者	王宣, 黄俊恺, 孔维恒, 王娟强, 张颖颖, 郭文萍, 赵文涛*,
作者单位	中国肉类食品综合研究中心; 中国海关科学技术研究中心
摘要	目的基于超高效液相色谱-串联质谱法建立植物替代蛋白产品中大豆过敏蛋白的检测方法, 开展植物替代蛋白产品中过敏蛋白的定量和真实性判定研究。方法大豆分离蛋白经胰蛋白酶水解后, 通过高分辨质谱采集数据并与Uniprot数据库匹配, 筛选出大豆特异性过敏蛋白多肽, 并采用响应面设计优化过敏蛋白提取条件, 从而构建过敏原定量方法。对市售植物替代蛋白产品进行实际样品检测, 初步得到不同植物替代蛋白产品中大豆过敏蛋白含量。结果筛选出线性较好的6种大豆定量多肽、相关系数均大于0.995, 方法检出限为0.182~2.080 μg/g、方法定量限为0.607~6.920 μg/g, 回收率为97.1%~126.6%, 日内精密度为5.17%~10.20%, 日间精密度为5.02%~11.70%。结论本方法特异性强、灵敏度高, 可以较好地针对植物替代蛋白产品进行检测, 实现了在无同位素标记肽段的情况下对大豆过敏蛋白的准确定量。在植物替代蛋白产品的过敏蛋白检测上具有较好的应用前景, 同时可实现植物替代蛋白产品的掺假鉴别及食品标签的过敏信息判别。
Abstract	Objective To establish a detection method for soybean allergens in plant-based protein products based on ultra performance liquid chromatography-tandem mass spectrometry, and conduct research on the quantification and authenticity determination of allergy protein in plant-based protein products. Methods After the soybean protein isolate was hydrolyzed by trypsin, the data were collected by high resolution mass spectrometry and matched with the Uniprot database to screen out the soybean specific allergen peptides. The response surface design was adopted to optimize the extraction conditions of the allergen protein, thereby constructing the quantitative method of allergens. Actual sample tests were conducted on commercially available plant-based protein products to preliminarily obtain the content of soybean allergy proteins in different plant-based protein products. Results The 6 kinds of soybean quantitative peptides with good linearity and correlation coefficients above 0.995 were selected. The limits of detection and the limits of quantification of the method were 0.182–2.08 μg/g and 0.607–6.920 μg/g. The spiked recoveries were 97.1%–126.6% and the intra-day and inter-day precisions were 5.17%–10.20% and 5.02%–11.70%. Conclusion This method has strong specificity and high sensitivity, and can well detect plant-based protein products, achieving accurate quantification of soybean allergens without isotope-labeled peptide segments. It has a good application prospect in the detection of allergen in plant-based protein products. Meanwhile, it can achieve the identification of adulteration in plant-based protein products and the discrimination of allergic information on food labels.
关键词	植物替代蛋白产品、大豆过敏蛋白、超高效液相色谱-串联质谱法、特征多肽、响应面优化
KeyWord	plant-based proteins; soybean allergens proteins; ultra performance liquid chromatography-tandem mass spectrometry; specific peptide; response surface optimization
基金项目
页码	59-68

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引用本文

王宣, 黄俊恺, 孔维恒, 王娟强, 张颖颖, 郭文萍, 赵文涛*. 基于大豆过敏蛋白的植物替代蛋白产品真实性研究 [J]. 食品安全质量检测学报. 2025; 16; (12). 59 - 68.

文献评论

张**东北农业****** 已认证✔

2025-11-04 07:40:43

该研究建立了基于超高效液相色谱-串联质谱法用于植物替代蛋白产品中大豆过敏蛋白的检测，在植物替代蛋白产品的过敏蛋白检测方面具有较好的应用潜力, 也为植物替代蛋白产品的掺假鉴别及食品标签的过敏信息判别提供了技术支撑。

刘**新疆维吾****** 已认证✔

2025-10-14 11:16:14

该研究针对植物替代蛋白产品大豆过敏蛋白检测与真实性判定的行业痛点，建立超高效液相色谱 - 串联质谱检测方法，选题贴合食品安全监管与过敏人群健康保障需求，应用价值明确。实验设计严谨，通过高分辨质谱结合数据库筛选出 6 种高特异性大豆定量多肽，经响应面优化提取条件，方法学验证显示相关系数＞0.995，检出限、回收率及精密度均达检测要求，且成功应用于 10 种市售样品检测，发现未标识过敏原信息样品，数据可靠且具实践意义。创新性体现在无需同位素标记肽段即可实现准确定量，弥补现有检测技术短板。整体而言，论文逻辑清晰、方法科学，为植物替代蛋白产品质量监管提供重要技术支撑，后续可进一步拓展方法适用范围。

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