Journal of Cell and Molecular Biology Research

Based on transcriptome sequencing data of Bletilla striata (B. striata), we used bioinformatics methods to analyze gene identification and sequence of PanK. First, we analyzed the isoelectric point, hydrophilicity and hydrophobicity, secondary and tertiary structure and domain of PANK protein in B. striata. Then we analyzed its subcellular localization, predicted phosphorylation site, signal peptide and transmembrane region. Finally, we built the evolutionary tree. The results showed that the PanK gene of B. striata contained an ORF of 2697 bp and encoded a total of 898 amino acids. The protein had a relative molecular mass of 99405.55 Da, which predicted to be a hydrophilic protein with an isoelectric point (pI) of 5.79. The protein had 11 threonine phosphorylation sites, and a conserved domain of pantothenate kinase (PLN02902 family protein), which contained 10 motifs. Then we found that this protein had no signal peptide and transmembrane region, and it was located in the cytoplasm through subcellular localization. The phylogenetic tree analysis showed that the PanK gene of B. striata was more closed to Dendrobium officinale (D. officinale). In conclusion, this research showed that thePanK gene had the structural characteristics of pantothenate kinase, and the sequence of amino and nucleic acid were highly similar to homologous species, which meant it was highly conservative in evolutionof homologous species. So we speculated that it played a role in the cytoplasm and might be related to the energy supply in cell metabolism.

Bletilla striata; PanK; Structure Characteristics; Functional Prediction