白及反式-肉桂酸4-单加氧酶基因C4H的序列分析-细胞与分子生物学研究-CSCIED科技核心评价数据库-手机版

白及反式-肉桂酸4-单加氧酶基因C4H的序列分析

Bioinformatic Analysis of Trans-cinnamate 4-monooxygenase Gene C4H in Bletilla striata

ES评分 0 浏览量：893 下载量：0

DOI	10.12208/j.ijcmbr.20210002
刊名	细胞与分子生物学研究 Journal of Cell and Molecular Biology Research
年，卷(期)	2021, 1(1)
作者	赵永彬,黄恻隐,徐程,钱露,李清清,何毅怀,徐德林*,
作者单位	遵义医科大学珠海校区广东珠海 ; 遵义医科大学细胞生物学教研室贵州遵义 ; 遵义医科大学附属医院贵州遵义 ;
摘要	反式-肉桂酸4-单加氧酶是植物中苯丙素和木质素合成的关键酶之一，研究C4H基因的序列信息对揭示其蛋白结构及功能具有重要的指导意义。从白及转录组数据库中分离得到BsC4H基因，运用生物信息学软件对该基因进行序列分析。分析得到的结果显示：该基因长度为1993 bp，编码的蛋白质长度为505 aa、分子量为58.01858 kD、理论等电点为9.20，该基因的二级结构主要由α螺旋结构和无规则卷曲结构组成，三级结构预测为单体结构。氨基酸序列与铁皮石斛C4H基因相似性最高，达95％，结构域分析发现该蛋白具有高度结构保守域，但并不包含有跨膜结构域和信号肽，亚细胞定位分析发现该酶被定位于内质网中。该研究结果为研究白及BsC4H基因提供了基础数据，也为研究其它物种C4H基因提供基础资料。
Abstract	Trans-cinnamic acid 4-monooxygenase is one of the key enzymes in the synthesis of phenylpropanoid and Lignin in plants. The C4H gene of Bletilla striata (BsC4H) was isolated from our former constructed transcriptome database and then analyzed by a series of bioinformatics software. The results showed that the length of the gene was 1993 bp, which encoding a protein with 505 aa in length which have molecular weight of 58.01858 kd and theoretical isoelectric point of 9.20. The predicted secondary structure of this protein was composed of helix structure and irregular coil structure, the tertiary structure was predicted to be monomer structure. BsC4H sequences of amino acid showed 95% similarity to C4H gene derived from Dendrobium officinale. The domain detection showed that BsC4H protein had a highly conserved domain, but no transmembrane domain and signal peptide. While the detection of subcellular localization found BsC4H was mainly functioned in the endoplasmic reticulum. The results of this study provided basic data for the subsequent study of BsC4H and developed inspirment for of C4H studies of other species.
关键词	白及；反式-肉桂酸4-单加氧酶；序列分析；结构预测
KeyWord	Bletilla striata; Trans-cinnamate 4-monooxygenase; sequence analysis; structure prediction
基金项目
页码	4-11

参考文献
相关文献

[1] 万大群, 赵仁全, 刘海, 等. 白及的成分、药理作用和临床应用研究进展[J]. 中国药业, 2017,26 (2):93-96.
[2] 沈金妤. 中药白及药理作用及临床应用进展[J]. 养生保健指南, 2018, (47):279.
[3] 董永成, 王启斌, 高蕾, 等. 中药白及外用制剂研究进展[J]. 中国药师, 2019, 22(1):133-136.
[4] 车玉红, 杨波, 郭春苗, 等. 榅桲C4H基因的克隆、序列分析及表达[J]. 经济林研究, 2020, 38(2):1-8.
[5] 李敏奇, 谢凌志, 陈永正, 等. 高通量筛选策略在细胞色素P450单加氧酶定向进化中的应用[J]. 合成化学, 2020, 28(4):371-384.
[6] 黄利娜, 吴光斌, 匡凤元, 等. 莲雾果实C4H基因的克隆及在NO处理下的表达分析[J]. 集美大学学报（自然科学版）, 2020, 25(2):105-112.
[7] Showkat Ahmad Bhawani, Nursuhailah Binti Suhaili, Rachel Marcella Roland，et al. Template Assisted Synthesis of Molecularly Imprinted Polymer for the Extraction of p-Coumaric Acid [J]. Asian Journal of Chemistry, 2020, 32(9):2342-2346.
[8] 张华玲, 刘绪, 杨春贤, 等. 甘薯肉桂酸-4-羟化酶基因克隆及序列分析[J].广西植物, 2018,38 (4):501-508.
[9] Delin Xu, Hongbo Chen, Murat Aci，et al. De Novo Assembly, Characterization and Development of EST-SSRs from Bletilla striata Transcriptomes Profiled throughout the Whole Growing Period. Plos ONE，2018,13(10): e0205954.
[10] 陈蒙, 刘海峰. 山葡萄C4H基因的克隆表达及遗传转化分析[J]. 西南大学学报（自然科学版）, 2019, 41(10):11-21.
[11] 黄利娜, 吴光斌, 匡凤元, 等. 莲雾果实C4H基因的克隆及在NO处理下的表达分析[J]. 集美大学学报(自然科学版), 2020, 25(2):105-112.
[12] 陈德伟, 王昕, 李红叶, 等. 芍药C4H基因的克隆及其在钙处理下的表达特性分析[J]. 分子植物育种, 2018, 16(9):2795-2801.
[13] 崔晓东, 王婷芬, 杜晶晶, 等. 苦荞α-螺旋发夹抗菌肽的分子改造抑制植物真菌活性[J]. 中国生物化学与分子生物学报, 2019, 35(2):196-204.
[14] Emmanuel Chekwube Ossai, Takashi Kuroiwa, Kaname Horikoshi, et al. Lipid Vesicle Preparation Using W/O/W Multiple Emulsions via Solvent Evaporation: The Effect of Emulsifiers on the Entrapment Yield of Hydrophilic Materials [J]. Journal of the American Oil Chemists Society, 2019, 96(12):1-12.
[15] Li B, Xie J, Yuan Z, et al. Mitigation of Inflammatory Immune Responses with Hydrophilic Nanoparticles. Angew Chem Int Ed Engl. 2018, 57(17):4527-4531.
[16] Rhys NH, Soper AK, Dougan L. Hydrophilic Association in a Dilute Glutamine Solution Persists Independent of Increasing Temperature. J Phys Chem B. 2015,119 (51):15644-15651.
[17] 刘宽亮, 赵志常, 高爱平，等. 芒果C4H基因的克隆及其表达分析[J]. 江苏农业科学, 2017, 45(14):8-12.
[18] Kong JQ, Lu D, Wang ZB. Molecular cloning and yeast expression of cinnamate 4-hydroxylase from Ornithogalum saundersiae baker. Molecules. 2014, 19(2):1608-1621.

引用本文

赵永彬,黄恻隐,徐程,钱露,李清清,何毅怀,徐德林*. 白及反式-肉桂酸4-单加氧酶基因C4H的序列分析 [J]. 细胞与分子生物学研究. 2021; 1; (1). 4 - 11.

文献评论

相关学者

相关机构